Dr. Stylianos was educated at Rutgers University and the New York University School of Medicine. He completed his General Surgical training at Columbia-Presbyterian Medical Center, and subsequently spent two years as the Trauma Fellow at the Kiwanis Pediatric Trauma Institute in Boston. Dr. Stylianos completed a Pediatric Surgical fellowship at the Boston Children's Hospital in 1992. Dr. Stylianos is Board-Certified in Pediatric Surgery, General Surgery and Surgical Critical Care. Dr. Stylianos joined the surgical staff of the Children's Hospital of New York in 1992, and is presently an Associate Professor of Clinical Surgery and Pediatrics at the Columbia Physicians and Surgeons. He developed and is the Director of the Pediatric Trauma Program at the Children's Hospital of New York, which was recently designated as one of two Regional Pediatric Trauma Centers in the New York metropolitan area. Dr Stylianos was named an Arnold P Gold Foundation Associate Professor of Surgery and has initiated multiple outreach, prevention, and education programs. Also, a hospital-wide bereavement program has been initiated and implemented with the support of the Arnold Gold Foundation and the Hospital. 

Dr. Stylianos' clinical specialties include neonatal surgery, surgical oncology, and minimal access surgery for children; he recently introduced the technique of video-assisted pectus excavatum repair at the Children's Hospital of New York. He organized and directed the 50-member team of physicians and nurses who separated conjoined twins at Children's Hospital of New York in 1993, 1995, and 2000.  Dr. Stylianos is currently the Medical Director of the Operating Room at the Children's Hospital of NewYork.

Dr. Stylianos served as Chairman of the Trauma Committee for the American Pediatric Surgical Association (APSA) for the past 5 years and authored the APSA position paper supporting all measures to reduce the toll of firearm violence in children. Dr Stylianos serves as the Co-Principal Investigator of the APSA Department of Health and Human Services, Maternal and Child Health Bureau Partnership for Information and Communication Grant (#HO3MC0006), "Partnership for Development and Disseminaton of Outcomes Measures for Injured Children." This project is funded from 1999-2004 with an annual award of $200,000 (Project total: $1 million). The initial work was the APSA Trauma Committee's multi-institution study, Evidence-based Guidelines for Resource Utilization in Children with Isolated Spleen and Liver Injuries, supported in part by an APSA Foundation supplemental Enrichment Grant. This work was presented at the APSA Annual Meeting in Rancho Mirage, CA, May 1999, and was published in the February 2000 issue of the Journal of Pediatric Surgery (JPS 35:164-169, 2000). The prospective phase of the study validated the proposed evidence-based guidelines at participating institutions. More than 300 children were enrolled in the prospective study to date. These data was presented at the 2001 APSA Annual Meeting in Naples, Fl and was published in the March 2002 issue of the Journal of Pediatric Surgery (JPS 37:453-456, 2002).